Address hepatotoxicity and drug induced liver injury (DILI) risks early on!
As the first of many R&D updates for 2024, we are thrilled to announce the expansion of our In vitro toxicology service portfolio and the launch of new research services focusing on hepatotoxicity assessment and drug-induced liver injury (DILI) evaluation. As a leading provider in the field of ADME-Tox, we understand the critical importance of timely assessment of hepatotoxicity risks early.
Robust hepatotoxicity assessment in pooled primary human hepatocytes with high-content imaging
Drug-induced liver injury, DILI, often leading to acute liver failure, can be caused by several mechanisms and poses a challenge in drug discovery. Our approach to assess and mitigate the risk for hepatoxicity and DILI utilizes automated high-content imaging, providing superior sensitivity and precision in the evaluation of steatosis, phospholipidosis and lysosomal trapping.
Steatosis and phospholipidosis
Steatosis, marked by the buildup of fatty acids and lipids, and phospholipidosis, a sign of lysosomal malfunction, may arise at concentrations of drugs lower than those causing cytotoxic effects, thereby increasing the risk for hepatotoxicity. Our tests employ pooled primary human hepatocytes to achieve complete metabolic function and acknowledging inter-individual variability in humans.
Lysosomal trapping
Certain types of compounds may be prone to lysosomal trapping or sequestration, which impacts their pharmacokinetics, efficacy, and safety properties, and is also one of the mechanisms inducing phospholipidosis. Our cutting-edge lysosomal trapping assay utilizes Lysotracker dye to evaluate the lysosomal sequestration potential of drug candidates. When the test compound undergoes lysosomal sequestration, there’s a concentration-dependent decrease in the lysosomal fluorescence signal as the compound competes with the dye, which accumulates into lysosomes.
Utilizing automated high-content imaging methods, the sensitive assays are coupled with rapid analysis and reliable readouts. Reach out to our team to discuss more!
Get in touch with our team!
With our cutting-edge methodologies, analytical equipment and scientific expertise, we are dedicated to helping our clients to success with their projects. Should you have any questions, please do not hesitate to reach out. We look forward to continuing our shared journey of innovation and success.
Let’s make molecules matter. Together.
